This site makes extensive use of JavaScript.
Please enable JavaScript in your browser.
Live
PTR
10.2.7
PTR
10.2.6
Beta
DNA
Post Reply
Return to board index
Post by
Livetoheal
Hello everyone!
First off, very very random topic, but appreciated if we could get some help.
Right, so my group is doing a Bio Lab, where we have to extract DNA. Our aim is to find out whether the quality ( price ) of the detergent used during the process has any effect on the end outcome of how much DNA you get.
What I'm wondering is how would one compare the results between the cheap used detergent, and the priced used one? We need to have an objective view of it, not subjective. So we can't just say "Oh well this one LOOKS like it has more DNA."
Thanks! =))
(If you're wondering how the process of extracting is being done
http://learn.genetics.utah.edu/content/labs/extraction/howto/
, it's pretty much that, except instead of using meat tenderizer, we're using pine-apple juice.)
Post by
tuckmuck203
Take a microscope and count the length of the individual strands/strand of DNA.
Measurement, while arbitrary, is objective.
Post by
Livetoheal
http://learn.genetics.utah.edu/content/labs/extraction/howto/images/Window_TestTube.jpg
The DNA will be looking like that. And we only have 2 hours with using this method in the lab. So I don't think we're going to have time to sit and count all the individual lengths of the DNA. But thanks I'll note down this idea anyway, as it can be used as an good example on how to make this lab a even more fair test..
Thanks for the response, keep em coming x)
Post by
124027
This post was from a user who has deleted their account.
Post by
Toldu
I don't know if there's enough DNA retrieved to even be weighable, but if you have access to a sensitive enough scale you could attempt to compare the weight of the DNA extracted. Make sure to weigh the sticks ahead of time so you don't have to attempt to remove the DNA from them; just do a comparison weighing.
Post by
tuckmuck203
I suppose you could measure it as a volume. Get some teaspoon or less measuring cups.
Post by
Monday
You live in Utah?
Post by
148723
This post was from a user who has deleted their account.
Post by
Livetoheal
Nope, Sweden. Btw, I'm not in University or anything, still college. Thanks for all the help, but I think this is a little too advanced for our level x)
Sorry should've written I'm not in Uni or something.
Post by
Monday
You live in Utah?
Probably not, University of Utah has some great genetics activities and stuff for high school bio teachers.
My teacher uses UU's site.
Oh, I see.
Post by
487010
This post was from a user who has deleted their account.
Post Reply
You are not logged in. Please
log in
to post a reply or
register
if you don't already have an account.